Sew Art: The Best Software for Converting Any Image into an Embroidery Design
A docent guided tour of collection highlights. Availability is limited to 15 participants and advance registration is recommended. A face mask/covering is encouraged. Same-day admission is included in the price of your tour ticket.
Sew Art Registration Serial 30
BC is still accepting applications for Spring 23 Semester. We have a wide variety of late-start classes open as well. Visit the SID for help with enrollment, registration, financial aid, lifting holds, ed. planning, transfer assistance, & more!
Multicolor fluorescence microscopy has become a key enabling technology in biology by providing the means to spectrally resolve cells, organelles, or molecules within tissues and to analyze their interactions. Strategies combining multiple distinct fluorescent labels are increasingly used to study spatial relationships among cells and molecules and to encode parameters such as neuronal connectivity1,2,3,4,5,6,7,8,9, cell lineage10,11,12,13,14,15, cycling state16,17, subtype identity18, genotype19,20, or signaling pathway activation21. Scaling up such approaches at the whole organ or tissue level would be a major step forward but is hindered by the lack of suitable large-volume multicolor microscopy methods. In recent years, serial block-face imaging, which relies on the automated, iterative alternation of imaging and microtome-based sectioning of a block of tissue, has been successfully transposed from electron microscopy to light microscopy22,23,24,25. This scheme has emerged as an effective means for generating data encompassing mm3-to-cm3 volumes of tissue at subcellular-resolution with either discrete or continuous sampling. One particularly active field of application is neuroscience, where block-face fluorescence imaging has opened the way to brain-wide mesoscale connectomics23,26 and single neuron reconstruction efforts26,27,28,29. However, microtome-assisted microscopy methods developed so far are limited to single- or dual-color imaging23,24,26,29. This limitation is due to the general difficulty of exciting a manifold of fluorescent proteins. In addition, achieving tissue-scale multicolor microscopic imaging requires addressing chromatic aberrations and channel registration over large volumes. These obstacles made it so far very difficult to probe cell interactions or more generally to image multiplexed or combinatorial fluorescent signals with micrometer-scale precision in samples exceeding a few hundreds of microns in depth.
Here, we report on a method for volume multicolor and multi-contrast microscopy with submicrometer registration of the image channels. Our approach, termed chromatic multiphoton serial (ChroMS) microscopy, relies on the integration of trichromatic two-photon excitation by wavelength mixing (WM)30 with automated serial tissue sectioning. We show that ChroMS microscopy delivers multicolor imaging over >mm3 volumes with constant micron-scale resolution and submicron channel coregistration, which sets new quality standards for large-scale multicolor microscopy. We demonstrate its performance for tridimensional imaging of mouse brains labeled by transgenic, electroporation-based or viral multicolor approaches. In addition to being perfectly suited for applications based on combinatorial fluorescence labeling, ChroMS microscopy also enables organ-wide imaging of label-free nonlinear signals such as third harmonic generation (THG) and coherent anti-Stokes Raman scattering (CARS). We illustrate its potential for high information-content three-dimensional (3D) imaging by demonstrating (i) analysis of astroglial cell morphology and contacts over several mm3 of cerebral cortex, (ii) color-assisted tracing of tens of pyramidal neurons in a densely labeled, mm-thick cortical sample, and (iii) brain-wide color-based multiplexed mapping of axonal projection trajectories and interdigitation.
Together, these results show that astrocytes present significant heterogeneity in the volume of their domains and in their local partitioning of the neural tissue, suggesting that they develop in a plastic rather than fixed manner and may adapt to both their local environment and neighbors during cortical development and maturation. Our findings illustrate the unique strengths of continuous multicolor 3D imaging with ChroMS microscopy for unbiased quantitative 3D analysis of cell morphology and contacts within large tissue volumes. Specific methodological advantages of ChroMS for that purpose include the following: (i) the color contrast increases the throughput of individual cell analysis for a given sample by alleviating the need to use very sparse labeling; (ii) it enables one to analyze contacts between cells labeled with different colors; (iii) the serial multiphoton approach suppresses the orientation and size artefacts due to section registration or tissue shrinking/swelling encountered respectively in histology and clearing techniques.
We present here a multicolor/multi-contrast tissue-scale microscopy method which bypasses the spectral limitation common to current large volume imaging techniques, and in particular expands the field of applications of serial two-photon microscopy23,44.
ChroMS microscopy achieves efficient one-shot excitation in three distinct spectral bands, enabling multicolor imaging of minimally processed tissue with sub-micron channel alignment and resolution preserved over cubic millimeter volumes. The integration of WM-excitation and serial block-face imaging solves two general problems encountered in tissue imaging: chromatic aberrations (inherent to all multicolor microscopy approaches) and inhomogeneous image quality across volumes. ChroMS microscopy can generate brain-wide atlas-like color datasets with subcellular resolution as well as continuous 3D datasets of supra-millimetric size with submicron multicolor precision and diffraction-limited axial resolution.
Image processing was done using Fiji64 (NIH,USA) and Matlab (Mathworks, USA), and 3D renderings were generated using IMARIS (Bitplane, Switzerland). Individual tiles were first batch cropped to remove scanning edge artifacts. Flat-field correction was then applied to individual tiles prior to stitching to correct for illumination inhomogeneity in the field of view. Illumination profiles were computed for each channel by averaging all the tiles of the channel and applying a large kernel Gaussian blurring. The tiles were then divided by the normalized (maximum intensity) illumination profiles and merged into RGB composites. Mosaic stitching was done using the Grid/Collection Stitching Fiji plugin65. Tile positions were computed using cross-correlation between tiles. For ChroMS continuous reconstructions, we used a custom version of the same plugin allowing 3D cross-correlation calculations between blocks. Of note, the high image quality provided over the entire acquisition depth by two-photon imaging permitted efficient 3D stitching without the need of a supplementary registration channel.
Chanel started using the date code system in the mid 1980's. We call them the social security numbers of the Chanel bags. Look inside the bag for a sticker with the serial number. The location of the serial number varies depending on the style of handbag and the year, but unless the bag is very vintage you should always find the date code inside.
The year the bag was made is determined by the amount of digits in the serial number, and the first digit(s) in that serial number. If your bag has 8 digits, it was produced between late 2005-current.
For 8 digit serial numbers, the year is determined by the first two digits (i.e. 24XXXXXX corresponds to late 2017-early 2018). If your bag has a 7 digit serial number, the year of production is determined by the first digit (i.e. 6XXXXXX corresponds to 2000-2002.)
If the serial number on a bag does not follow the Chanel formula or has more than 8 digits it is most certainly a fake. In rare cases you can find vintage Chanel bags with a 6-digit serial number. Most bags made in 1986 had a 7-digit number starting with zero, but in very early 1986 bags the leading zero was skipped where a 6-digit number started with a 1 or 2.
Of course, the Chanel formula is readily available information, so advanced counterfeiters could create a serial number that fits the table above. Pay attention to the font, the sparkles and the x cutout in the hologram. You need to examine the serial number sticker more carefully: it will never be a plain, boring sticker that looks out of place.
If you looked everywhere inside the bag and still find no serial number, it's not the end of the world! Remember, these are stickers that are constantly rubbing against the bag's contents, and they can fall off and fade with use. It could also be a vintage authentic Chanel crafted prior to 1986.
Singers have always been marked with serial numbers ever since production began. Each number is corresponding to a model from a certain date, and even location. All Singer sewing machines up until 1900 have no letter prefix, and came from all around the world. The Singer company eventually managed their production from all their factories to match up with the serial number flow.
Hey mindy..I recently got an antique singer and it also has a serial number that begins with a G followed by 7 numbers. Were able to find anything to help you identify the manufacturing year on yours?
I picked up an old singer at a odd and ends shop. The serial number, which is located on the top under the gold Singer emblem. I do not see it on your chart. The number is AD745900. Is that the serial number and if it is could you tell me the age? It is in beautiful and looks similar to the model on your website above, the one with the red fabric.